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KMID : 1007520110200020445
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Food Science and Biotechnology
2011 Volume.20 No. 2 p.445 ~ p.454
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Optimization of β-Carotene Production by a Mutant of the Lactosepositive Yeast Rhodotorula acheniorum from Whey Ultrafiltrate
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Mohammad Reza Nasri Nasrabadi
Seyed Hadi Razavi
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Abstract
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The present work investigated on carotenogenesis with high β-carotene content by a new isolated highactivity strain-producer Rhodotorula acheniorum mutant MRN in cheese whey ultrafiltrate. After a serial of UV, ethymethanesurfonate (EMS), and nitrosoguanidine (NTG) mutagenesis, a mutant named MRN of the red lactosepositive yeast strain R. acheniorum was obtained. Then, the effects of different growth medium factors on carotenoid production by this mutant at batch-scale level were identified and optimized by means of response surface methodology (RSM) in order to achieve high-level production of β-carotene. The optimum conditions required to achieve the highest level of β-carotene (262.12±1.01 mg/L) were determined as follows: whey ultrafiltrate (WU) lactose concentration 55 g/L, pH 5.85, ammonium sulfate concentration 3.5 g/L, temperature 23oC, and aeration rate 1.56 vvm. The medium optimization resulted in a 6.45-fold increase in volumetric production (262.12±1.01 mg/L) and a 4.62-fold increase in the cellular accumulation (10.69± 0.19 mg/g) of β-carotene.
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KEYWORD
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Rhodotorula acheniorum, whey ultrafiltrate, β-carotene, mutagenesis, response surface methodology
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